Meta-analysis of Indonesian Octopus laqueus Kaneko & Kubodera 2005 (Cephalopoda: Octopodidae) using Mt-DNA COI as Genetic Marker

O. laqueus was first discovered not long ago in 2005 the Ryuku Islands, Japan. Its geographical distribution and molecular identification are therefore still rarely. Nucleotide sequences based on mt-DNA COI for that have been uploaded GenBank until before this study carried out were only six sequences. Since DNA barcoding of has some advantageous characteristics, aimed to analyse genetic difference Indonesian data available GenBank. Samples collected 2019 - 2020 from Karimunjawa (n=16) Bangka-Belitung (n=2). The extracted using 10% chelex methods, PCR amplified Folmer’s primer sequenced Sanger methods. Pairwise alignment distance MEGA-X, whereas phylogenetic tree reconstructed Bayesian BLAST resulted 685 bp with a range 92,07-99,24 percentages identity. mean pair-wise distances within-clade 0,002 0,006, whilst between clade 0.0883. Combining suggestion ITF current, it is concluded taken raised same species as those Malaysia (MN711655) Japan (AB302176). Specimens suggested came different species, they separated into second by 8.83%. One single sample (AB430543) which laid outside two clades 11.63%-11.38% also represent species. Overall, opens various further studies other loci markers.